Part:BBa_K2045004
MelA P334S-Mutant tyrosinase gene heightened expression
iGEM Concordia has showcased the synthesis of nanoparticles through many chemical and plant based methods. iGEM Concordia wanted to find an innovative application where we will be able to develop a system where we can synthesize and attach nanoparticles through a single biological cell system. MelA is a gene that produces Eumelanin, which is a dark pigment. Eumelanin has carboxyl and hydroxyl quinone groups, whose charges help reduce gold ions, present in the cytoplasm, into gold nanoparticles. In order to accomplish this, we used iGEM Cambridge’s part, BBa_K274001, for nanoparticle production. In order to make more gold nanoparticles within the E.coli cell, iGEM Concordia IMPROVED the part in order to have higher expression of MelA for higher gold nanoparticle synthesis.This is done by a point mutation, (Santos CNS, Stephanopoulos G., 2007). Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 1332
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 1184
Illegal BsaI.rc site found at 489
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